Estimation of genetic polymorphism in cultivars, lines and mutants of garden pea (Pisum sativum L.) using DNA-markers on the basis of retrotransposons

IRAP-method was used for study of insertional polymorphism of Ту1-copia retrotrans-posons in 16 cultivars, lines and mutants of garden pea (Pisum sativum L.). 10 combinations of primers distinguishing polymorphism in analyzed lines were designed. 72 polymorphic markers were found. Using IRAP-analysis genetic distances were counted and comparative dendrogramm reflexing genetic similarity and relationship between samples analyzed. Individual IRAP-spectrums were analyzed for every cultivar and line; some cultivar-specific fragments suitable for identification and marking of analyzed forms were found. Usage of IRAP-analysis for such purposes in pea cultivars and lines was shown may be prospective.