Evaluation of the efficiency of DNA isolation from different parts of the false flax plant Camelina sativa (L.) Crantz

The results of the evaluation of the effi-ciency of DNA isolation from different parts of the false flax (Camelina sativa (L.) Crantz) plants using an automated station are presented in this article. The aim of the work was to adapt the method of DNA extraction to obtain DNA of sufficiently high quality suitable for molecular genetic studies such as PCR and genetic cer-tification. Dried seeds, 7-day-old seedlings and leaves of false flax were used as plant material. We performed DNA isolation using a magnetic particle-based reagent kit and the automated station. We evaluated the quality and quantity of DNA by spectrophotometry and aga-rose gel electrophoresis, and tested its suitability for PCR using SSR markers. The results showed that the highest yield of DNA with the lowest contamination was obtained when isolated from leaves. However, de-spite the low purity of the samples obtained from seeds and seedlings, they were all suitable for PCR analysis. For express diagnostics and genetic certification, seeds are the most convenient material since they are availa-ble in large quantities and do not require additional preparation. The study confirmed the efficiency of the automated method of DNA isolation for genetic stud-ies of false flax, which is particularly important for large-scale genetic diversity projects.