Evaluation of the toxic effect of aminoglycoside antibiotics on the meristematic tissue of the roots of onion

Автор: Kantsavaya Irina, Alekseenko Olga

Журнал: Бюллетень науки и практики @bulletennauki

Рубрика: Биологические науки

Статья в выпуске: 3 т.6, 2020 года.

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In the work examines the impact of aminoglicoside antibiotics (amicacin, gentamicin, canamicin, streptomycin) and cytokinin 6-BAP on the mitotic activity of cells meristematic tissue of the roots of the onion. Research methods: Allium-test, cytogenetic analysis, statistical analysis. In variants of the experiment using 6-BAP at concentrations of 5.0 mg/l, tested with aminoglycoside antibiotics (gentamicin in concentrations of 50.0 and 100.0 mg/l, and streptomycin in concentrations of 150.0 and 1000.0 mg/l) when microscopic drugs observed in apical meristem in 68.8-88.0% of the appendage roots of onions lack division. Their mitotic index was close to zero and most of the cells were in a profatestate condition. It was established that the tested concentrations of aminoglycoside antibiotics suppressed mitotic activity in the meristematic tissue of the appendages of the onion roots. Considerably, in 1.6-2.5 times MI reduced in variants of application of 6-BAP, gentamicin, streptomycin in concentrations of 1000.0 mg/l, amicacin and canamicin. Increased concentration of gentamicin from 50 mg/l to 100.0 mg/l, streptomycin from 150.0 mg/l to 1000.0 mg/l, canamicin from 100.0 mg/l to 100.0 mg/l doesn’t have a significant negative effect on mitotic activity of meristem cells.

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Allium-тест

Короткий адрес: https://sciup.org/14116017

IDR: 14116017   |   DOI: 10.33619/2414-2948/52/06

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