Determination of optimal doses of ionizing radiation for inactivation of the causes of necrobacteriosis

The study of the radioresistance of the culture of Fusobacterium necroforum strain 8TS630501 was carried out in the Department of Radiobiology of the Federal State Budget Scientific Institution "FCTRBS-ARRVI". The causative agent of necrobacteriosis was irradiated with the Researcher gamma installation, source - 60Co with an exposure dose rate of 3.7 kGy/hour in dose ranges from 5 to 30 kGy, with an inter-dose interval of 5 kGy. The viability of the irradiated culture was determined by plating on a nutrient medium and staging a bioassay in laboratory animals. Studies have established that complete inactivation of the causative agent of necrobacteriosis occurs when irradiated in the intervals from 25 to 30 kGy. Radiation exposure at a dose of 5 kGy does not affect the growth of this culture. Inhibition of the growth of irradiated material at doses of 10, 15 and 20 kGy is directly dependent on the degree of radiation exposure, which is confirmed by bioassay in laboratory animals.