Optimization of conditions for obtaining plasmid DNA of E. coli with fragments of genes encoding immunodominant proteins of the ASF virus

African swine fever (ASF), due to its contagiousness and mortality, raises serious concerns about the well-being of the global pig industry. Successful prevention and eradication of ASF is hampered by many factors, including the globalized nature of livestock production and the lack of specific prophylaxis. Development of effective responses to ASF is essential in combating current epidemics and associated trade restrictions. Particular scientific interest is presented by the search for immunodominant antigens of the ASF virus capable of ensuring the formation of protective immunity. In this study, the screening and selection of genes encoding highly conserved immunodominant proteins of the ASF virus was carried out, their phylogenetic analysis was carried out, the conditions for cloning genes and cultivation of transformed E. Coli were optimized in order to produce plasmid DNA in preparative quantities. The approaches we propose make it possible to obtain purified plasmid DNA preparations in sufficient quantities for further assembly of vectors based on adeno-associated virus (AAV), which, in turn, will form the basis of an effective and safe strategy for creating a candidate vaccine against ASF. Taking into account the properties of the proteins under consideration, it can be assumed that a future vaccine developed on the basis of the p72, pp60, p54, p30 antigen complex may be applicable not only in Russian Federation, but also in the territories of neighboring countries of Eastern Europe.