Optimization of condition for PCR amplification of the BoLA-DRB3-gENE locus

BoLA DRB3 is the immune response gene of an organism whose alleles are associated with resistance or sensitivity to bovine leukemia. Methods for BoLA typing by sequencing (SBT) with decipherment of the entire exon 2 DRB3 are known in the art. The aim of the work was to optimize the conditions for PCR amplification of the BoLA-DRB3 gene locus, to a level suitable for further procedure of typing by sequencing. As a result of the studies, the PCR conditions for amplification of the BoLA-DRB3 gene locus with the DRB3FRW & DRB3REV primers and the "Encyclo Plus PCR kit" reagents were optimized to ensure the effective production of a specific reaction product.