Charactristics of cytokines, phenotipic features and neutrophilsphagocytosis activity in asthmatic patients during exacerbation

The aim of our study was to measure the level of IL -8, IL-6, IL-4, IL-17 depending on spontaneous or induced release by peripheral blood leukocytes in patients with bronchial asthma exacerbation with comparative analysis of asthmatics' blood neutrophils phenotypic features. The obtained data indicate that the level either pro-inflammatory or anti-inflammatory cytokines was increased in patients with asthma exacerbations and significant increase of concentration of CD45+CD66b+CD11b+ cells in comparing with healthy donors. We determined that during asthma exacerbation the concentration of CD45+, CD66b+, CD11b+ blood granulocytes almost doubled in comparing with healthy donors. Also there is a significant increasing of blood granulocytes' phagocytosis activity. We observed the elevated level of spontaneous production of inflammatory mediators. Moreover, the stimulation of cytokines synthesis and release in patients with asthma does not always lead to increase of induced level of cytokines in contrast to healthy donors.