Evaluation of acute-phase proteins in the conditions of reparative regeneration

Dynamics of changes in the content of serum protein fractions was studied in experimental intramedullary osteosynthesis by using fixators, coated with titanium and hafnium nitrides in rabbits. Serum protein fractions were determined before surgery and after surgery at a period of 5 to 180 days. At a constant level of total protein after induced bone trauma and during the osteoregeneration process, a change in the serum protein spectrum, characteristic of the inflammatory reaction was observed. Revealed changes were characterized by a two "waves" response of the organism. The First wave was developed by the 5th day and was characterized by a decrease in the level of albumin and an increase in α - and β2 - globulins level. The attenuation of this reaction in the experimental group occurred at an earlier date than in the comparison group. The second wave reached its maximum by the 180th days and was characterized by an increase in β2 - and γ - globulins level. These changes correspond to the early phases of the non-specific response which includes a higher synthesis of positive and a lower synthesis of negative acute-phase proteins by the liver under the influence of cytokines, and to a later specific adaptive immune response. The less expressed intensity of the reactive changes in rabbits of the experimental group, as opposed to the comparison group, indicates that implants coated with titanium and hafnium nitrides don’t provoke pronounced damaging effects.