Peculiarities of toxic effects exerted by hexyl ether of 5-aminolevulinic acid and grounds for working our regulations of its safe production and application

Our research object was hexyl ether of 5-aminolevulinic acid applied as plants growth regulator which was synthesized with an original technology by Bioorganic Chemistry Institute of Belarus National Academy of science. Our research goal was to determine peculiarities of toxic effects exerted by this new plants protector on experimental models in vivo/in vitro and to give grounds for hygienic standards of its contents in various media which were to provide its safe production and application. We conducted our research with the use of toxicological, physiological, hematological, biochemical, immunologic, cytogenetic, cytological, and statistical methods. We were the first to perform toxicological assessment of this new plants growth regulator under different regimes, doses and ways of introduction into laboratory animals' bodies and it helped us to detect its toxicometric parameters and peculiarities of its biological effects which became apparent through skin-resorptive and cumulative properties, irritating impacts on mucous tunics, moderate reproductive toxicity without substantial signs of gonadotropic, mutagenic, and allergenic impacts on a body. We detected that toxic impacts exerted by the examined substance on a body was combined with membrane-tropic and cytotoxic effects. We determined criteria and limiting parameters of hazardous effects exerted by hexyl ether of 5-aminolevulinic acid in the course of a chronic experiment, and it gave us grounds for fixing allowable daily dose for a man and for working out a number of regulations on the substance contents in environmental objects (working area air, water, and soil), in food raw materials, and in food products (grain, rape, rape and linen oil). The obtained results were used as a basis for fixing 9 hygienic standards and were used for the state registration of this plant growth regulator; it will provide its safe production and application in agriculture.