Certification of new sunflower lines and hybrids of VNIIMK breeding by means of biochemical and molecular markers

Certification of new promising sunflower lines and hybrids on isozyme markers and molecular microsatellite loci is an important method of breeders’ rights protection. The purpose of this work was to certify 12 Unes and four hybrids of sunflower of VNIIMK breeding. To characterize the breeding material there were used methods of isozyme analysis and polymerase chain reaction of DNA amplification. Using five isozyme and eight SSR-loci, biochemical and molecular-genetic passports were done. A set of the new lines revealed by analysis of variability of the isozyme systems became a less unique in comparison to sunflower Unes of VNIIMK breeding studied in previous years. An analysis of biochemical characteristics of 12 lines showed that they can be united into eight groups differed from one another. The hybrid combinations possessed unique isozyme spectra. Singularity of the lines and hybrids showed by analysis of isozyme systems was 75%. At analyzing of SSR- loci an individuality of allele set for each line and hybrid was determined, except lines VKl-imi A and VKl-imi В being the sterile and fertile analogs. A singularity of the lines and hybrids obtained by means of microsatelUte loci was 93%. The polymorphic and informative systems for this group of samples are determined to be four isozyme systems: esterase, malatdehydrogenase, 6-phosporus-gluconatdehydro- genase, glucosephosphatisomerase, and five microsatelUte loci: ORS509, ORS 595, ORS 1144, ORS 1796, and ORS 1036. An average number of aUeles for locus for them were 2.