The first Russian experience of using PCA3 and TMPRSS2-ERG for prostate cancer diagnosis

Over the past 20 years a large number of prostate cancer (PCa) markers have been proposed. A combination of PCA3 and TMPRSS2-ERG is among them. The real-time RT-PCR- based method for РСА3 and TMPRSS2-ERG determination in urine and prostate tissue is being developed at Russian Scientific-research Institute of Urology. Purposely to assessment of its diagnostic value, two independent samples of prostate tissue (n=78) and urine (n=56) specimens were investigated. The blood level of PSA, pathomorphological findings, PCA3 and TMPRSS2-ERG urinary and tissue expression were assessed. Samples were divided into groups, according to pathomorphological findings: PCa and non-cancerous prostatic changes (BPH and chronic prostatitis). Prostate tissue РСА3 expression was significantly higher in PCa patients (Ме РСА3 in tissue 3,1 (1,2:4,9)) compared to BPH and chronic prostatitis patients (Ме РСА3 in tissue 9,1 (6,9:10,4)) (р