Real-time PCR for diagnosis of bovine parainfluenza-3

The aim of the work was to determine the effectiveness of the PCR method in the diagnosis of bovine parainfluenza-3 (BPI-3). For research, we used the reference strain SF-4, the vaccine strain PTK-45/86 and the isolate LD-9 of the parainfluenza virus 3. As a result of the studies, the effectiveness and high execution speed of RT-PCR with fluorescence detection for the diagnosis of bovine parainfluenza type 3 were established. All studied strains of BPIV-3 showed positive amplification.