The primary screening of fungal strains antagonists from a collection of the biological methods laboratory in VNIIMK to a phoma rot on sunflower. Part I

There are presented data of the primary screening of fungal strains antagonists from a collection of the biological methods lab of the V.S. Pustovoit AllRussian Research Institute of Oil Crops (VNIIMK) to an aggressive isolate of the Phoma rot pathogen (Phoma macdonaldii Boerema) affecting sunflower by a method of double or opposite crops in vitro. We estimated 29 fungal strains from the collection on the three nutrient mediums (potato-sucrose agar (PSA), Rudakov’s and oat agar (OA). We stated 23 of them possessing antagonistic activity with one or some types of action mode against Phoma pathogen on sunflower. 21 strains of fungi-antagonists possessed double mode of action (competition for nutrient area and hyperparasitism), five of them (SM-1 Sordaria sp., T1, T-2, T-3 Trichoderma sp. and Pbc-1 Penicillium brevi-compactum) were active on three nutrient mediums. Colonizing activity of these strains was 80.0- 100% of nutrient medium surface, at hyperparasitic zone size of 1.0-5.0 сm2 . Maximal hyperparasitic zone were marked for the strains: A-1 Basidiomycetes (43.4 сm2 ) on PSA; Pk-1 P. vermiculatum (15.0 сm2 ) on Rudakov’s nutrient medium; Tr-1 Trichothecium roseum (14.2 сm2 ) on PSA; Pbc-1 P. brevicompactum (12.0 сm2 ) on OA. Maximal sterile zone to a Phoma pathogen was marked for starins: Xk-3 Chaetomium sp. (12.5 mm) on PSA and An-1 Aspergillus niger (10.0 mm) on ОА.