Choice of optimal methodology for DNA isolation from mycelium of Alternaria species for PCR

The different methods of DNA isolation from mycelium of fungi of Alternaria Nees species cultivated on different mediums were compared. The optimal appeared to be DNA isolation by means of commercial reagents QIAGEN Dnseay Plant MiniKit (Germany). The method of Zolan and Pukkila (1986) modified with application of AkO3 at the stage of homogenization is also suitable. There are isolated six ISSR-primers initiating amplification of polymorphous DNA fragments in Alternaria Nees species which are observed on sunflower in Krasnodar region. These ISSR-loci can be used for studying of inter- and intraspecific variability of fungi of Alternaria species.