Polymorphism of TP53 (RS1042522) gene and peculiarities of the immune profile in children exposed to airborne benzo(a)pyrene

Examining peculiarities of an immune profile and genetic polymorphisms is especially relevant when identifying markers of effect and sensitivity to exposure to benzo(a)pyrene in northern areas. We examined 1253 children who lived in industrial centers and on conditionally clean territories in the north and south of Eastern Siberia. Levels of benzo(a)pyrene in ambient air and in children’s blood were determined by using HPLC. TP53 (rs1042522) polymorphism was examined by using real-time PCR; p53 levels were identified with flow cytofluorometry; IgG to benzo(a)pyrene, by the radioallergosorbent tests. Exposure of children in Northern Siberia to airborne benzo(a)pyrene at the dose of 7.11·10-3 µg/(kg·day) causes benzo(a)pyrene contamination in blood, activates apoptosis (p53) and stimulates occurrence of specific sensitization (IgG to benzo(a)pyrene) (p -3 µg/(kg·day). The detected changes in immune profiles of children living in Northern Siberia are associated with G-allele and GG-genotype (rs1042522) of the TP53 gene (OR = 1.37-1.83, p -3 µg/(kg·day) in Northern Siberia bears some signs of activated apoptosis (p53) and specific sensitization (IgG to benzo(a)pyrene) associated with G-allele and GG-genotype of the TP53 gene (rs1042522) (OR = 1.37-1.83, p -3 µg/(kg·day) in Southern Siberia, which are associated with C-allele and CC-genotype of the same gene (OR = 1.55-2.38, p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }