Obtaining emulsified probiotic preparation Bacillus subtilis and its effect on the intestinal microflora of laboratory rats

Studied the use a new form probiotic preparation B. subtilis in emulsion bacteria in palmitic acid. In assessing the effectiveness of probiotics, it was assumed that the bacteria DNA content in feces is proportional to their content in the intestine. Analysis of the number bacteria DNA was carried out by PCR in real time. The results showed a positive effect of the use emulsified bacteria. This was seeing in increase amount of B. subtilis in the intestine and as a consequence of their DNA in rat feces. Increasing the number of bacteria B. subtilis was accompanied by decrease the number of Bifidobacterium sp., Lactobacillus sp. and E. coli in the intestine. The ability of bacteria B. subtilis to have a positive effect on the growth rate of rats, the absence of pathogenicity and toxicity was shown.