The obtaining regenerants of Medicago glutinosa L. resistant to oil pollution without use of the selective agent

Basing on a somaclonal variability, the regenerants of Medicago glutinosa L. resistant to petroleum pollution were selected in vitro by an optimized scheme without an addition of selective agent to the media for induction of callus and regenerants. Such parameters as a survival rate for 56 days and plant height of the regenerants and the intact plants were compared. In 20 % of the regenerated plants the resistance was lower and in 65 % it was the same as compared with the control, and 15 % of the regenerated plants demonstrated a greater increase comparing to the other plants. It was shown regenerants contributed to a better purification of soil, polluted with petroleum as compared to intact plant grown from seeds.