Obtaining the recombinant -l-fucosidase (AlfB)
Автор: Kiryanova S.V., Cherenkov D.A., Tolkacheva A.A., Korneeva O.S.
Журнал: Вестник Воронежского государственного университета инженерных технологий @vestnik-vsuet
Рубрика: Биотехнология, бионанотехнология и технология сахаристых продуктов
Статья в выпуске: 3 (57), 2013 года.
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In this study the gene α-L-fucosidase (AlfB), amplified by PCR was cloned in the vector plasmid pet23b + sites for EcoR1 - Xho1. Gene expression occurred in the cells of Escherichia coli top 10 under the control of the T7 promoter. The strain-producer recombinant protein α-L-fucosidase was obtained, optimum values of pH and temperature was determined by optimizing the conditions of the enzyme action.
Α-l-фукозидаза, escherichia coli
Короткий адрес: https://sciup.org/14040070
IDR: 14040070
