Possibilities of regulating antioxidant activity of medicinal plant extracts

Possibilities of regulating antioxidant activity of medicinal plant extracts

Автор: Potoroko I.U., Kalinina I.V., Naumenko N.V., Fatkullin R.I., Shaik S., Sonawane S.H., Ivanova D., Kiselova-kaneva Y., Tolstykh O., Paymulina A.V.

Журнал: Человек. Спорт. Медицина @hsm-susu

Рубрика: Спортивное питание

Статья в выпуске: 4 т.17, 2017 года.

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The aim of the paper. The aim of the present paper is to evaluate the effectiveness of extracting biologically active substances of antioxidant action from plant raw materials on the basis of applying different extraction approaches. Materials and methods. We did the chemical research of BAS extracts Lonicera сaerulea L. and Urtica folia L. concerning their migration parameters during fraction division (honeysuckle berries) and changing extraction conditions (dried nettle leaves). Freeze-drying of raw material (by means of a freeze-drying apparatus “Ineye-6” under the following conditions: temperature -50 ± 5 °C, pressure no more than 6 Pa) was applied to preserve the bioactivity in a honeysuckle fraction. The experiment included a wide range of chemical parameters - antioxidant activity (АОА), mass fraction of extractives, phenol compounds, anthocyanins and vitamins. The UFLC Shimadzu Corporation Pump: LC-20AD Detector: SPD-M20A Prominence Diode array detector was used to identify bioactive substances. Results and discussion. The paper studies the influence of various approaches concerning bioactive compounds extraction from Lonicera сaerulea L. and Urtica folia L. The application of ultrasound (US) with frequencies 22 ± 0.6 kHz increases the effectiveness of extraction process. It helps to conduct extraction at low temperatures (60 ± 2 °С) without using chemical reagents. The paper presents the results of water and ethanol extraction obtained from different fractions of honeysuckle and dried nettle leaves. It also provides the results of antioxidant activity analysis of this extraction and a total of antioxidant activity. Urtica folia L extracted by US of 120 W (2,4043 ± 0,084 mg/ml) and a sample based on the water-ethanol extraction agent without an ultrasonic bath (2,5209 ± 0,032 mg/ml) showed higher antioxidant activity. We defined the technological parameters of obtaining a dry extract of Lonicera сaerulea L by means of ultrasound and freeze-drying for preserving the maximum bioactive compounds of raw material (up to 92-85 %). Conclusion. The results of the experiment proved that ultrasound-assisted extraction improves the extraction kinetics and the output of bioactive compounds from the substrate. The extraction technology excludes the infusion stage which reduces energy intensity.

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Medicinal plants, extraction, biologically active compounds, antioxidant activity, ultrasound

Короткий адрес: https://sciup.org/147153377

IDR: 147153377   |   DOI: 10.14529/hsm170409

Список литературы Possibilities of regulating antioxidant activity of medicinal plant extracts

  • Afanas'ev, I.B. Chelating and free scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation/I.B. Afanas'ev, A.I. Dorozhko, A.V. Brodskii, V.A. Kostyuk//ZBiochem. Pharmacol. -1989. -Vol. 38. -P. 1763-1769.
  • Amarowicz, R. Antioxidative and radical scavenging effects of phenolics from Vicia sativum/R. Amarowicz, A. Troszyńska, R.B. Pegg//Fitoterapia. -2008. -Vol. 79. -P. 121-122.
  • Andriy, G. Development and validation of a RP-HPLC method for the simultaneous estimation of luteolin and apigenin in herb of Achillea millefolium/G.Andriy//Pharma Innovation. -2013. -No. 2. -P. 7-14.
  • Ashokkumar, M. Hot topic: Sonication increases the heat stability of whey proteins/M. Ashokkumar, J. Lee, B. Zisu, R. Bhaskarcharya//Journal of Dairy Science. -2009. -No. 92. -P. 5353-5356.
  • Борисова, М.С. Лечение клюквой, брусникой, черникой/М.С. Борисова СПб.: Изд-во «Литера», 2003. -58 с.
  • Brown, J.E. Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties/J.E. Brown, H. Khodr, R.C. Hider, C.A. Rice-Evans//Biochem. -1998. -Vol. 330. -P. 1173-1178.
  • Dadali, V.A. Biologically active substances of medicinal plants as a factor of detoxification/V.A. Dadali, V.G. Makarov//The supply issues, 2003, No. 5. -P. 49-55.
  • Elim, H.-J. Evaluation of antioxidant activity of vetiver (Vetiveria zizanioides L.) oil and identification of its antioxidant constituents/H.-J. Elim, F. Chen, X. Wang, H.Y. Chung, Z. Jin//J. Agric. Food Chemistry. -2005. -Vol. 53. -P. 7691-7695.
  • Fatkullin, R. Application of ultrasonic waves for the improvement of particle dispersion in drinks/R. Fatkullin, N. Popova, I. Kalinina, V. Botvinnikova//Agronomy Research. -2017. -No. 15. -P. 1295-1303.
  • Головина, Е.Ю. Содержание антиоксидантов в листьях некоторых растений семейства бобовые г. Калининграда/Е.Ю. Головина, Т.А. Брахнова//Тезисы докладов VIII международной конференции «Биоантиоксидант». Москва, 4-6 окт. 2010 г. -М.: РУДН, 2010. -558 с.
  • Голуб, О.В. Формирование качественных характеристик сброженного напитка на основе меда растительного сырья/О.В. Голуб, Г.П. Чекрыга, О.К. Мотовилов//Пиво и напитки. -2015. -№ 5. -С. 26-30.
  • Материалы 6-й Международной конференции «Фитотерапия, биологически активные вещества естественного происхождения в современной медицине». -Черноголовка, 2006. -С. 260-268.
  • Katalinic, V. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols/V. Katalinic, M. Milos, T. Kulisic, M. Jukic//Food Chemistry. -2006. -Vol. 94. -P. 550-557.
  • Киселева, Т.Ф. Выявление предпосылок комплексной переработки плодово-ягодного сырья сибирского региона/Т.Ф. Киселева, И.С. Зайцева, Д.Б. Пеков, Н.В. Бабий//Техника и технология пищевых производств. -2009. -№ 3. -С. 7-11.
  • Корулькин, Д.Ю. Природные флавоноиды/Д.Ю. Корулькин, Ж.А. Абилов, Р.А. Музычкина, Г.А. Толстиков. -Новосибирск: Тео, 2007. -232 с.
  • Krasulya, O. Applications of sonochemistry in Russian food processing industry/O. Krasulya, S. Shestakov, V. Bogush, I. Potoroko//Ultrasonics Sonochemistry. -2014. -No. 21. -P. 2112-2116.
  • Masaki, H. Active-oxygen scavenging activity of plant extracts/H. Masaki, S. Sakaki, T. Atsumi, H. Sakurai//Biol. Pharm. Bul. -1995. -Vol. 18. -P. 162-166.
  • Melidou, M. Protection against nuclear DNA damage offered by flavonoids in cells exposed to hydrogen peroxide: the role of iron chelation/M. Melidou, K. Riganakos, D. Galaris//Free Radic. Biol. Med. -2005. -Vol. 39. -P. 1591-1600.
  • Naumenko, N.V. Sonochemistry effects influence on the adjustments of raw materials and finished goods properties in food production/N.V. Naumenko, I.V. Kalinina//Materials Science Forum. -2016. -Vol. 870. -P. 691-696.
  • Okubo, T.N. In vivo effects of tea polyphenol intake on human intestinal microflora and metabolism/T.N. Okubo, N. Ishinara, A. Oura, M. Serit//Biosci. Biotechnol. Biochem. -1992. -Vol. 56. -P. 588-591.
  • Pietta, P.-G. Flavonoids as antioxidants/P.-G. Pietta//J. Nat. Prod. -2000. -Vol. 63. -P. 1035-1042.
  • Потороко, И.Ю. Практические аспекты использования ресурсоэффективных технологий в пищевых производствах как фактора качества готовой продукции/И.Ю. Потороко, Ю.И. Кретова, И.В. Калинина//Товаровед продовольственных товаров. -2014. -№ 10. -С. 8-13.
  • Rice-Evans, C.A. Structure antioxidant activity relationships of flavonoids and phenolic acids/C.A. Rice-Evans, N.M. Miller, G. Paganda//Free Radic. Biol. Med. -1996. -Vol. 20. -P. 933-956.
  • Rice-Evans, C.A. The relative antioxidant activities of plant-derived polyphenolic flavonoids/C.A. Rice-Evans, N.J. Miller, P.G. Bolwell, P.M. Bramley//Free Radic. Res. -1995. -Vol. 22. -P.
  • Shanmuga, S.R. Validation of RP-HPLC Method for Simultaneous Determination of Apigenin and Luteolin in Ethanol Extract of Clerodendrum serratum (Linn.) Leaves/Shanmuga Sundaram Rajagopal, Babitha K Vazhayil, Liz Varghese, Mahadevan Nanjaian Development//Asian Journal of Applied Sciences. -2017. -No. 05 (01). -P. 52-60.
  • Шатилов, А.В. Роль антиоксидантов в организме в норме и при патологии/А.В. Шатилов, О.Г. Богданова, А.В. Коробов//Ветеринарная патология. -2007. -№ 2. -С. 207-211.
  • Щербаков, Д.Л. Особенности влияния адреналина на перекисное окисление липидов в миелокариоцитах зрелых и старых крыс in vitro/Д.Л. Щербаков, В.В. Емельянов, В.Н. Мещанинов//Вестник Урал. мед. академ. науки. -2013. -№ 4 (46). -С. 102-105.
  • Щербаков, Д.Л. Антиоксидантное действие триптофана и никотиновой кислоты в головном мозгу крыс разного возраста при иммобилизационном стресс-воздействии/Д.Л. Щербаков, В.В. Емельянов, В.Н. Мещанинов//Успехи геронтологии. -2014. -Т. 27. -№ 4. -С. 730-736.
  • Shestakov, S. A New Look at Cavitation and the Applications of Its Liquid-Phase Effects in the Processing of Food and Fuel/S.Shestakov//Applied Physics Research. -2012. -Vol. 4. -P. 19-29.
  • Shestakov, S. Mathematical Model of the Spatial Distributing of Density of Erosive Power of Multibubble Cavitation/S. Shestakov, V. Babak//Applied Physics Research. -2012. -No. 4. -P. 64-77.
  • Silva, M.M. Structure-antioxidant activity relationships of flavonoids: a reexamination/M.M. Silva, M.R. Santos, G. Caroco, R. Rocha//Free Radic. Res. -2002. -Vol. 36 -P. 1219-1227.
  • Skerget, M. Solubility of ß-carotene and oleic acid in supercritical carbon dioxide and data correlation by a density based model/M. Skerget, Z. Knez, M. Habulin//Fluid Phase Equil. -1995. -No. 109. -P. 131-138.
  • Spencer, J.P.E. The small intestine can both absorb and glucuronidate luminal flavonoids/J.P.E. Spencer, G. Chowrimootoo, R. Choudhury, E.S. Debnam//FEBS Lett. -1999. -Vol. 458. -P. 224-230.
  • Spencer, J.P.E. Cellular uptake and metabolism of flavonoids and their metabolites: implications for their bioactivity/J.P.E. Spencer, M.M.A.E. Mohsen, C. Rice-Evans//Arch. Biochem. Biophys. -2004. -Vol. 423. -P. 148-161.
  • Tian-yang WangBioactive flavonoids in medicinal plants: Structure, activity and biological fate/Tian-yang Wang, Li. Qing, Bi. Kai-shun//Asian Journal of Pharmaceutical Sciences. Available online 15 August 2017.
  • Усов, А.И. Полисахариды водорослей/А.И. Усов, Г.П. Смирнова, Н.Г. Клочкова//Биоорган. химия. -2001. -Т. 27. -№ 6. -С. 444-448.
  • Vitaly Roginsky. Chain-breaking antioxidant activity of natural polyphenols as determined during the chain oxidation of methyl linoleate in Triton X-100 micelles/Vitaly Roginsky//Archives of Biochemistry and Biophysics. -2003. -No. 15. -P. 261-270.
  • Vostаlovа, J. Lonicera caerulea fruits reduce UVA-induced damage in hairless mice/J. Vostаlovа, A. Galandаkovа, I. Palikova, J. Ulrichova//Journal of Photochemistry and Photobiology B: Biology. -2013. -No. 5. -P. 1-11.
  • Wu L.-C. Antioxidant and antiproliferative activities of red pitaya/Wu L.-C., Hsu H.-W., Chen Y.-C., Chin C.-C., Lin Y.-I., Ho J.A.//Food Chem. -2006. -Vol. 95. -P. 319-327.
  • Wu, Jia-Jiuan Wang Extraction of antioxidative compounds from wine lees using supercritical fluids and associated anti-tyrosinase activity/Wu, Jia-Jiuan, Jung-Chuan Lin, Chih-Hung//JSF. -2009. -No. 50. -P. 33-41.
  • Xiao, Q.C. RP-HPLC-DAD determination of flavonoids: separation of quercetin, apigenin and luteolin in Marchantia convolute/Q.C.Xiao, B.X. Jian//Iranian Journal of Pharmaceutical Research. -2009. -No. 54. -P.175-181.
  • Заворохина, Н.В. Моделирование напитков социальной направленности с учетом специфики региона/Н.В. Заворохина//Пиво и напитки. -2013. -№ 6. -С. 8-12.
  • Заворохина, Н.В. Растительное сырье уральского региона для производства безалкогольных напитков/Н.В. Заворохина, М.П. Соловьева, О.В. Чугунова, Е.В. Пастушкова, В.В. Фозилова//Пиво и напитки. -2013. -№ 4. -С. 28-31.
  • Zheng, M. DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide/M. Zheng, X. Wang, L.J. Templeton, D.R. Smulski//Journal of Bacteriology. -2001. -Vol. 183. -P. 4562-4570.
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