Практическое применение массового параллельного репортерного анализа в биотехнологии и медицине

Практическое применение массового параллельного репортерного анализа в биотехнологии и медицине

Автор: Романов Станислав Евгеньевич, Лактионов Петр Павлович

Журнал: Клиническая практика @clinpractice

Рубрика: Научные обзоры

Статья в выпуске: 4 т.13, 2022 года.

Бесплатный доступ

Развитие и жизнедеятельность организма опирается на тканеспецифичные генные программы. Ключевую роль в регуляции таких программ играют регуляторные элементы генома, а нарушения в их работе могут приводить к развитию различных патологий, включая пороки развития, онкологические и аутоиммунные заболевания. Развитие высокопроизводительных геномных исследований привело к появлению методов массового параллельного репортерного анализа (МПРА), которые позволяют проводить функциональную проверку и идентификацию регуляторных элементов в масштабе генома. Изначально МПРА применялся в качестве инструмента для исследования фундаментальных аспектов эпигенетики, однако этот подход также имеет огромный потенциал для клинической и практической биотехнологии. На текущий момент МПРА используют для валидации клинически значимых мутаций, идентификации тканеспецифичных регуляторных элементов, поиска наиболее перспективных для интеграции трансгена локусов. МПРА является также незаменимым инструментом для создания высокоэффективных экспрессионных систем, спектр применения которых распространяется от подходов для наработки белков и конструирования суперпродуцентов терапевтических антител нового поколения до генной терапии. В настоящем обзоре предложены к рассмотрению основные принципы и области практического применения методов высокопроизводительного репортерного анализа.

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Массовый параллельный репортерный анализ, мпра, экспрессионные системы, биотехнология, клиническая биоинженерия

Короткий адрес: https://sciup.org/143179874

IDR: 143179874   |   DOI: 10.17816/clinpract115063

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