Primary metabolites and betanin: their interplay in the roots of table beet (Beta vulgaris L.)

Автор: Sokolova Diana V., Solovieva Alla E., Shelenga Tatyana V.

Журнал: Овощи России @vegetables

Рубрика: Агрохимия, агропочвоведение, защита и карантин растений

Статья в выпуске: 2 (70), 2023 года.

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Relevance. The main source of the natural pigment betanin is table beet, known for its medicinal and antioxidant properties, earliness and long shelf life, rich in bioactive compounds, minerals and vitamins. This research was induced by the lack of information required by breeders to increase betanin content in beet. Metabolite profiling is an effective way to assess the interplay between individual metabolites and betanin content in table beet. Materials and methods. The material was selected from the the N.I. Vavilov Institute of plant industry (VIR) collection. Biochemical analysis was based on VIR’s guidelines, and metabolite profiling on gas chromatography, coupled with mass spectrometry. Results. 17 free amino acids were found in the beet root extract. The greatest number of positive correlations with other amino acids (r>0.72) was found in tyrosine, alanine and phenylalanine. A significant (r = -0.66) negative correlation was observed between betanin and succinic acid, credibly associated with betalamic acid. Sucrose predominated among carbohydrates (95%). Sucrose and maltose showed a weak positive correlation with betanin. Unsaturated oleic and saturated palmitic acids dominated among fatty acids (52% and 20% of total fatty acids, respectively). Earlier-ripening and cold-resistant table beet accessions showed a predominance of unsaturated fatty acids and lower betanin content. The disclosed interactions are important for betanin-rich red beet breeding


Red beet, betalains, betanin, free amino acids, organic acids, carbohydrates, fatty acids

Короткий адрес:

IDR: 140297791   |   DOI: 10.18619/2072-9146-2023-2-54-64

Список литературы Primary metabolites and betanin: their interplay in the roots of table beet (Beta vulgaris L.)

  • Шачек Т.М., Плитко Т.Ю., Севостьянов С.М. Разработка способа получения натурального красителя из свеклы. Научные стремления. 2017;(21):35-39.
  • Herbach K.M., Stintzing F.C., Carle R. Stability and color changes of thermally treated betanin, phyllocactin, and hylocerenin solutions. Journal of Agricultural and Food Chemistry. 2006;(54):390-398.
  • Aztatzi-Rugerio L., Granados-Balbuena S.Y., Zainos-Cuapio Y., Ocaranza-Sánchez E., Rojas-López,M. Analysis of the degradation of betanin obtained from beetroot using Fourier transform infrared spectroscopy. Journal of Food Science and Technology. 2019;56(8):3677-3686.
  • Cai Y., Sun Mei, Wu H., Huang R., Corke H. Characterization and quantification of betacyanin pigments from diverse Amaranthus species. Journal of Agricultural and Food Chemistry. 1998;(46):2063-2070.
  • Castellanos-Santiago E., Yahia E.M. Identification and quantification of betalains from the fruits of 10 mexican prickly pear cultivars by high-performance liquid chromatography and electrospray ionization mass spectrometry. Journal of Agricultural and Food Chemistry. 2008;(56):5758-5764.
  • Stintzing F.C., Schieber A., Carle R. Rote Bete als farbendes Lebensmitteleine Bestandsaufnahme. Obst Gemuse Kartoffelverarbeitung. 2000;85(5/6):196−204.
  • Henriette M.C. Betalains: properties, sources, applications, and stability. Intern. Journal of Food Science and Technology. 2009;(44):2365-2376.
  • Буренин В.И., Лудилов В.А., Соколова Д.В. Комплексное исследование генофонда столовой свеклы. Картофель и овощи. 2016;(2):39-40.
  • Kapadia G.J., Azuine M.A., Sridhar R., Okuda Y., Tsuruta A., Ichiishi E., Mukainake T., Takasaki M., Konoshima N.H., Tokuda H. Chemoprevention of DMBA-induced UV-B promoted, NORI-induced TPA promoted skin carcinogenesis, and DEN-induced phenobarbital promoted liver tumors in mice by extract of beetroot. Pharmacological Research. 2003;(47):141-148.
  • Lechner J.F., Wang L.-S., Rocha C.M., Larue B., Henry C., McIntyre C.M., Riedl K.M., Schwartz S.J., Stoner G.D. Drinking water with red beetroot food color antagonizes esophageal carcinogenesis in N -nitrosomethylbenzylaminetreated rats. Journal of Medicinal Food. 2010;13(3):733-739.
  • Gandia-Herrero F., Escribano J., Garcia-Carmona F. Biological activities of plant pigments betalains. Critical Reviews in Food Science and Nutrition. 2016;(56):937-945.
  • Butera, D., Tesoriere, L., Di Gaudio, F., Bongiorno, A., Allegra, M., Pintaudi, A. M., … Livrea, M. A. Antioxidant Activities of Sicilian Prickly Pear (Opuntia ficus indica) Fruit Extracts and Reducing Properties of Its Betalains: Betanin and Indicaxanthin. Journal of Agricultural and Food Chemistry. 2020;50(23):6895-6901.
  • Slavov A., Karagyozov V., Denev P., Kratchanova M., Kratchanov C. Antioxidant activity of red beet juices obtained after microwave and thermal pretreatments. Czech journal of Food Sciences. 2013;(31):139-147.
  • Da Silva D.V.T., Pereira A.D., Boaventura G.T., Ribeiro R.S. de A., Verícimo M.A., Carvalho-Pinto C.E. de, … Paschoalin V.M.F. Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats. Nutrients. 2019;11(9):1978.
  • Hayakawa K., Agarie S. Physiological roles of betacyanin in a halophyte, Suaeda japonica Makino. Plant Production Science. 2010;(13):351-359.
  • Nakashima T., Araki T., Ueno O. Photoprotective function of betacyanin in leaves of Amaranthus cruentus L. under water stress. Photosynthetica. 2011;(49):497-506.
  • Jain G., Schwinn K.E., Gould K.S. Betalain induction by l-DOPA application confers photoprotection to saline-exposed leaves of Disphyma australe. New Phytologist. 2015;207(4):1075-1083.
  • Brockington S.F., Walker R.H., Glover B.J., Soltis P.S., Soltis D.E. Complex pigment evolution in the Caryophyllales. New Phytologist. 2011;190(4):854-864.
  • Polturak G., Grossman N., Vela-Corcia D., Dong Y., Nudel A., Pliner M., Levy M., Rogachev I., Aharoni A. Engineered gray mold resistance, antioxidant capacity and pigmentation in betalain-producing crops and ornamentals. Proceedings of the National Academy of Sciences. USA. 2017;(114):9062-9067.
  • Tzin V., Galili G. New insights into the shikimate and aromatic amino acids biosynthesis pathways in plants. Molecular Plant. 2010;3(6):956-972.
  • Sakuta M. Diversity in plant red pigments: Anthocyanins and betacyanins. Plant Biotechnology Reports. 2014.8(1):37-48.
  • Esatbeyoglu T., Wagner A.E., Schini-Kerth V.B., Rimbach G. Betanin - A food colorant with biological activity. Molecular Nutrition & Food Research. 2015;59(1):36-47.
  • Strack D., Vogt T., Schliemann W. Recent advances in betalain research. Phytochemistry. 2003;(62):247-269.
  • Sasaki N., Abe Y., Wada K., Koda T., Goda Y., Adachi T., Ozeki Y. Amaranthin in feather cockscombs is synthesized via glucuronylation at the cyclo-DOPA glucoside step in the betacyanin biosynthetic pathway. Journal of Plant Research. 2005;118:439-442.
  • Соколова Д.В., Соловьева А.Е. Перспективный исходный материал для селекции сортов свеклы с высоким содержанием бетанина. Аграрная Россия. 2019;(8):26-32.
  • Ермаков А.И., Арасимович В.В., Ярош Н.П.; ред. А.И. Ермаков. Методы биохимического исследования растений. Ленинград. 1987. С.63-91.
  • Pucher G.W., Curtis L.C., Vickery H.B. The red pigment of the root of the beet (Beta vulgaris). A method to determine betanin. Journal of Biological Chemistry. 1938;(123):71‒76.
  • Kjeldahl J. Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern. [New Method for the Determination of Nitrogen in Organic Substances.] Zeitschrift für analytische Chemie. 1883;(22):366-383.
  • Perchuk I., Shelenga T., Gurkina M., Miroshnichenko E., Burlyaeva M. Composition of primary and secondary metabolite compounds in seeds and pods of asparagus bean (Vigna unguiculata (L.) Walp.) from China. Molecules. 2020;25(17):3778.
  • Worley B., Powers R. Multivariate Analysis in Metabolomics. Current Metabolomics. 2012;1(1):92-107.
  • Shtark O.Y., Puzanskiy R.K., Avdeeva G.S., Yurkov A.P., Smolikova G.N. et al. Metabolic alterations in pea leaves during arbuscular mycorrhiza development. Peer J. 2019;(7):1-33.
  • Ruiz-Hernández V., Roca M.J., Egea-Cortines M., Weiss J. A comparison of semi-quantitative methods suitable for establishing volatile profiles. Plant Methods. 2018;(14):67.
  • Wang M., Lopez-Nieves S., Goldman I.L., Maeda H.A. Limited tyrosine utilization explains lower betalain contents in yellow than in red table beet genotypes. Journal of Agricultural and Food Chemistry. 2017;(65):4305-4313.
  • Соколова Д.В. Эколого-географическое изучение накопления бетани- на у перспективных образцов столовой свеклы коллекции ВИР. Труды по прикладной ботанике, генетике и селекции. 2019;180(4):66-74.
  • Тимакова Л.Н., Борисов В.А., Фильрозе Н.А., Успенская О.Н., Соколова Л.М. Оценка качества сортов свеклы столовой в условиях Московской области. Картофель и овощи. 2020; 7: 28‒32.
  • Соколова Д.В., Шеленга Т.В. Соловьева А.Е. Сравнительная характе- ристика биохимического состава образцов мангольда и столовой свеклы коллекции ВИР. Овощи России. 2019;5(49):77-83.
  • Bhagyalakshmi N., Thimmaraju R., Narayan M.S. Various hexoses and dihexoses differently influence growth, morphology and pigment synthesis in transformed root cultures of red beet (Beta vulgaris). Plant Cell, Tissue and Organ Culture. 2004;(78):183-195.
  • Hou Q., Ufer G., Bartels D. Lipid signaling in plant responses to abiotic stress. Plant Cell and Environment. 2016;39(5):1029-1048.
  • Дударева Л.В., Рудиковская Е.Г., Шмаков В.Н. Влияние низкоинтенсив- ного излучения гелий-неонового лазера на жирнокислотный состав кал- лусных тканей пшеницы (Triticum aestivum L.) Биологические мембраны. 2014;31(5):364-370.
  • Жуков А.В. Пальмитиновая кислота и ее роль в строении и функциях мембран растительной клетки. Физиология растений. 2015;62(5):751-760.
  • Badea C., Basu S.K. The effect of low temperature on metabolism of membrane lipids in plants and associated gene expression. Plant Omics Journal. 2009;2(2):78-84.
  • Нохсоров В.В., Дударева Л.В., Петров К.А. Состав и содержание липи- дов и их жирных кислот в хвое Pinus sylvestris L. и Picea obovata Ledeb. при закаливании к низкой температуре в условиях криолитозоны Якутии. Физиология растений. 2019;66(4):286-294.
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