Application of high-performance liquid chromatography-high resolution tandem mass-spectrometry for determination of pharmaceuticals in natural water samples

A practical method for determination of pharmaceuticals (caffeine, ketoprofen, diclofenac, Ciprofloxacin) in natural water samples has been established. The solid-phase extraction (Oasis HLB cartridges) and method of high-performance liquid chromatography with high resolution tandem mass-spectrometry (LTQ OrbiTrap, ThermoFinnigan) is reported. The method has been tested on standard solutions. Detection limit (5-10 ng/l), stable detection threshold and recovery for standard solutions have been estimated. The results of the analysis of the standard solutions on different TOF mass-spectrometers (MX 5310, IAI RAS and LCMS-IT-TOF, Shimadzu) have been described.