Applicability of the method of fluorimetric detection and classical liquid chromatography in the determination of mycotoxins in various matrices

Mycotoxins are an important group of chemical compounds in economic terms, but at the same time they are extremely diverse in structure and properties which makes it impossible to use any one method to search for them and quantify them in different matrices. Given the impact of these compounds on living organisms, often leading to mass intoxication and death of farm animals, poisoning among food consumers, the importance of finding means of decontamination of raw materials and substrates is obvious. At the first stage of this process, the mycotoxin content in various matrices is searched for and quantified. It is necessary to note the variety of existing methods that allow determining the concentration of mycotoxins with different accuracy and efficiency. The method of high-performance liquid chromatography has taken an important place among others due to its versatility and high accuracy. The purpose of the research is to consider the possibilities of one of the methods which allow determining with high accuracy the content of the most economically important samples due to the high toxicity, variety of effects caused and wide prevalence of mycotoxins: aflatoxins, ochratoxins, zearalenone, fumonisin - HPLC method with fluorimetric detection (FLD). The tasks of the research are: analysis of existing methods for determining mycotoxins and variations of these methods, factors affecting the quality of determination and reproducibility of the results. The HPLC-FLD method in various versions is quite indicative. It has high accuracy and repeatability of the result which can be used in scientific research. This is its scientific novelty.