Protein components proteolysis of plant raw materials with high allergenic potential

The paper presents new data on the state of the problem of gluten intolerance. Data on the effectiveness of the biocatalytic effect on allergenic proteins of grain raw materials are given. The results of studies of the activities of hydrolytic enzyme preparations for the destruction of high-protein polymers-allergens of grain raw materials were obtained. The fractional composition of samples of grain raw materials and their hydrolysates was studied and it was found that the selected enzymes obtained on the basis of the filamentous fungus Aspergillus oryzae, and the enzyme system consisting of enzyme preparations obtained on the basis of filamentous fungi Aspergillus oryzae and Aspergillus niger, makes it possible to effectively modify allergenic fractions to low molecular weight peptides and amino acids. A decrease in the level of molecular masses of hydrolysates from 97 to 30-25 kDa was revealed. The composition of free amino acids in the obtained hydrolysates was studied and it was found that, as a result of enzymatic lysis, there is a significant increase (from 2.5 to 9 times) in such amino acids as serine, glutamine, glycine and proline. A comparative evaluation of the action of the enzyme preparation Amyloprotoorisin and an enzyme system containing enzymes of amylolytic and proteolytic action was carried out, and it was found that the presented enzyme preparations can be used to obtain low molecular weight wheat hydrolysates. These hydrolysates will be used to formulate gluten-free products.