Development of biotechnological method of creating the low-temperature stress resistant forms of coriander in vitro

The influence of low temperature stress on the development of embryogenic callus and isolated embryoids in vitro in coriander was studied and sublethal freezing regimes were established. A greater resistance to cold stress at the embryoculture in comparison with calli which kept the temperature decrease up to -10 °С (within 18 days) was revealed, whereas the screening of zygotic embryos may be conducted when the temperature decreases to -12 °С with more extended exposition (up to 27 days). The correlation between the winter hardiness of genotype and the main indicators of embryoculture development (correlation coefficients up to 0.76-0.96) was found. This demonstrates the possibility of in vitro selection the forms, resistant to low temperatures. The scheme of breeding of the coriander forms with increased resistance to cold stress, using callus cultures and isolated zygotic embryos, has been developed.