Development of methods of long-term storage of cultures Corynebacterium pseudotuberculosis

Caseous lymphadenitis of sheep is widespread in Western Siberia farms. Among Merino and crossbred sheep affected to 24,0% of the population. In the zones of strong and significant lesions often circulated in the exciter R- and I-forms, as in zones of weak and moderate S-forms predominate. The causative agent of caseous lymphadenitis (pseudotuberculosis sheep) - C. pseudotuberculosis - Gram-positive, polymorphic, fixed, aerobic bacterium size 0,5-3,1 mm in length and 0,5-0,9 microns in width, do not form spores and capsules. Corynebacterium remain viable during freeze-drying for 15 years, while maintaining over 12 years of their original properties. At the speed of the withering away of Corynebacterium cells in the process of freeze-drying, and church-nenii the following factors: the high vacuum of 30,7%, the low temperature of 13,9%, 10,8% protection Wednesday, the residual moisture of 10,6% and terms 28,6%. A proven way to save us Corynebacterium strains used in the manufacture of specific biological products for the prevention and control of caseous lymphadenitis of sheep and dried strains deposited in VGNKI veterinary drugs (Moscow) can serve as a benchmark for research and educational purposes. Permanent crops and crops subcultures to study their properties and maintain biological activity led to the deaths of some strains and phenotypic changes in their properties. Storage cultures in semisolid agar 0,25% vaseline oil layer under at +4? C proved unreliable because they do not allow to maintain the culture in the form of R- more than 12 months. While S-forms is stored and can longer time (19 months). The research allowed to choose the optimal modes liofi-tion, choose the cryoprotective medium to preserve and freeze-dried cultures for 20 years (time of observation), and deposit in C. rseudotuberculosis VGNKI vet drugs Moscow. During storage (12 years), two strains R - lost form glycolytic and catalase activity and acquired phenotypic traits S-forms.