Development of complex enzyme preparation producer for processing starch-containing waste from pressure-type filters in the production of baker's yeast

Lack of use of innovation technology with more efficient raw material processing leads to a large amount of industrial waste while producing bakery compressed yeast. Waste from the production of the yeast industry includes: setting after clarification of molasses (residuum), de-yeast wort, scourage and yeast in the form of waste on pressure-type filters. Yeast crop as waste on pressure-type filters that can be used for feed purposes is about 1,5% by weight of the produced yeast, and the main component of the waste is starch. For its processing the authors screened microorganisms-producers and selected a genetically competent stock culture capable of synthesizing target enzymes (glucoamylase, ?-amylase, pullulanase) - Rhisopus tritici t1 (Rh. Oryzae t1) with activity of enzymes 100,0; 8,4 and 20,0 fU/ml, respectively. There have been conditions developed for the liquid-phase cultivation of the producer, that provide the maximum biosynthesis of target enzymes and the production of a complex enzyme preparation for processing starch-containing waste from pressure-type filters in the production of baker's yeast. It was noted that starch, dextrin and maltose are the best carbon sources for the producer of Rhisopus tritici t1. The rational composition of the medium, providing the maximum values of the synthesized enzymes, includes, %: starch - 16, potassium nitrate – 0,8, corn-steep extract – 0,85. Activity of enzymes – glucoamylase, alpha-amilase and pullulanase - increased in 96-120 hours of cultivation by 18,0%; 19,0% and 50,0%, respectively.