Development of technologies for identifying risks of reducing biosafety of grain raw materials

The strategy of scientific and technological development of the Russian Federation in the long-term period defines the most significant priorities for the country's development. Among the major challenges is the provision of food security and food independence of the country. Global climate change is making adjustments to ensure the sustainability of the agro-industrial complex. With the reduction of food resources, timely reorientation of new areas of activity is necessary. In a set of effective activities, new technological solutions in the field of biosafety of plant raw materials and processed products are essential. Mycotoxin (MT) contamination of food products is a global problem of the present day, for the Russian Federation the most known MT producers are toxigenic molds of the genera Aspergillius, Penicillium, Fusarium and Alternaria. The most dangerous metabolites produced by the common mold fungi Aspergillus flavus or A. parasiticus deserve special attention due to their prevalence, as well as their hepatotoxic and carcinogenic properties. The purpose of the study is to develop technologies for reliable identification of factors of biosafety violation of grain raw materials on the basis of microbiome visualization methods in combination with assessment of climatic conditions of cultivation and prognostic research. The material presents studies confirming the presence of toxigenic mucoromycetes and predicts the risks of their secondary metabolites accumulation. New approaches for obtaining evidence of the possibility of the presence of secondary metabolites (SM) and the formation of masked forms of micromycetes are presented. For these purposes, imaging and FT-IR spectroscopy methods were applied to investigate the functional groups determining the risks. The scientific novelty of the obtained data is due to the application of new hybrid approaches for the identification of risks of biosafety violation of grain masses under the conditions of global climate change.