Reference intervals of microelements concentration in the cattle testes with paratypic factors consideration

The purpose of the study is to calculate the reference intervals for the concentration of trace elements in the testes of bulls, taking into account the influence of paratypical factors. The object of the study is the concentration of zinc, iron and manganese in the testes of Hereford bulls bred in the Krasnozersky, Maslyaninsky and Novosibirsk Districts of the Novosibirsk Region and Tselinny Districts of the Altai Region. Testis samples were taken from 31 clinically healthy animals aged 16-18 months. Determination of the concentration of chemical elements in the samples was carried out using the method of atomic absorption spectrometry with electrothermal atomization. The homogeneity of dispersions in groups was assessed using the Fligner-Keelin test. The Kruskal-Wallis method was used to reveal the influence of paratypical factors. Intergroup comparisons were made using Dunn's test with Holm's correction. The median values of the content of zinc, manganese and iron in the testes of livestock were in the range of 11.9-15.4, 0.35-0.50 and 16.4-26.1 mg/kg, respectively. The level of phenotypic variability in the content of the studied trace elements in bulls from the Maslyaninsky District was significantly higher than in cattle from other areas. Group dispersions for zinc concentration in the testes of bulls are homoscedastic (χ2 = 5.42; P = 0.14). The influence of paratypic factors on the level of zinc in the testes of animals was revealed (Н = 16.35; p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }