The role of hydrogen sulfide and carbon monoxide in collagen-induced platelet aggregation in patients with coronary artery disease

Purpose of research. To study the effect of hydrogen sulfide and carbon monoxide donors on collagen-induced platelet aggregation in healthy donors and patients with coronary artery disease.Introduction. There is evidence that the hydrogen sulfide (H2S) and carbon monoxide (CO) gas transmitters play an essential role in regulating the functional activity of various organs and systems of the human body. According to previous reports, CO and H S are considered inhibitors of platelet aggregation.Material and Methods. A total of 37 participants were examined during the study. The control group included 13 healthy donors; group of patients with coronary artery disease comprised 24 people. All examined patients received regular combination baseline therapy in accordance with modern recommendations for the treatment of coronary artery disease. Platelet aggregation activity was studied by a turbidimetric method using a two-channel laser analyzer (220 LA Biola, Russia). The degree and rate of platelet aggregation were determined in platelet-rich plasma based on the curves of light transmission and the average size of aggregates as markers of the functional activity of platelets. Collagen at a final concentration of 2 mg/mL was used to induce platelet aggregation. Sodium hydrosulfide (NaHS) was used as a hydrogen sulfide donor. Carbon monoxide-releasing molecule-II (CORM-II) was used as a carbon monoxide donor. Experimental data are presented as Me (Q1 -Q3). Wilcoxon T-test was used to test the homogeneity of paired or dependent samples. Values were considered tatistically significant when p was function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }