Screening of sunflower lines and op varieties of VNIIMK breeding for ALS-inhibitor resistance

The main task of the research was to estimate the frequency of a dominant gene for resistance against herbicides in the oil bearing germplasm of VNIIMK. Wide screening for Pulsar and Express resistance of sunflower lines and open pollinated varieties of VNIIMK breeding was done. The research has been carrying at central experimental base of VNIIMK (Krasnodar) and Armavir experimental station (Armavir). Open pollinated varieties Rodnik, Master and Oreshek as well as lines VK276, VK678, VA6, VA769, VK580, VK585 and VA384 were used. No resistant plants were obtained after observation on 680000 plants of three OP varieties and seven lines grown on three experimental fields with total area of 15 ha treated with imidazolinones or sulfonylurea herbicides. DNA markers were used for molecular identification of herbicide resistance genes. DNA was extracted from the leaves of individual plants according to Saghai-Maroof methods. Particularly electrophoretic spectra of DNA fragments were estimated. It was amplificated with allele specific primers for the ImiSun gene and allele specific primers for the CLHA-Plus gene. Frequency of a dominant gene for resistance against imidazolinones was estimated less than 5 х 10 -6 (1:200000) for germplasm of OP varieties and lines of VNIIMK and less than 4х10 -6 (1 : 280000) for lines of Armavir research station. Frequency of a dominant gene for resistance against sulfonylurea was estimated less than 5 х 10 -6 (1 : 200000) for germplasm of VNIIMK lines.