Nuclear DNA content in rice (Oryza sativa L.) regenerants derived from anther culture in vitro

Rice is an important food crop grown in the south of the Russian Far East. Therefore, breedingnew varieties with high harvest and crop quality is relevant. Anther in vitro culture is successfully applied in breeding programs in rice-growing countries, including Russia. In antherin vitro culture, flow cytometry is applicable to select haploid, dihaploid and polyploidregenerants.Cytological studies show genome variations from haploids to hexaploids in plant tissue in vitro culture, and also chromosome changes which result in aneuploidy or endopolyploidyleding to an inconstant nuclear DNA content. In the work, we followed the aims i) to evaluated nuclear DNA content by flow cytometry in anandrogenic rice regenerant population, and ii) to estimate the applicability of the combination of two approaches, the anther in vitro culture technique and flow cytometry, in rice breeding. A total of 1099 regenerantsfrom in vitro anther culture of a single F2(UkrNIIS 3435 x Ukr 96) rice ( Oryza sativa L...