Developing biopolymeric scaffolds for regenerative tissue genesis

Development and introduction of new biotechnological analogues of tissues and organs into clinical practice, in particular human tissue-engineered skin equivalents, designed for temporal or permanent replacement of damaged or destroyed tissue, remains an urgent problem of modern medicine. One of the most promising approaches for tissue reconstitution is an application of bioderived materials such as biodegradable 3D matrixes. Combining collagen extraction, gelation of collagen as flat matrixes, culturing HaCaT keratinocytes on the formed matrixes with subsequent fixation and decellularization allowed to develop nonliving acellular dermo-epidermal skin analogues consisting of two main componentscollagen and laminin. The in vivo evaluation of the skin scaffolds transplanted onto fullhickness skin wounds created on the back of rats showed enhanced wound healing. a new protein scaffold consisting of two componentscollagen and laminin of basal membrane mimicking the structure of human skin is proposed for implementation in regenerative medicine.