Creation of expression vectors with recA gene from E. coli for the purpose induction of recombination in plants

Taking into account current models of recombination in eucaryotes the authors make an attempt to use recA gene from E.coli, protein product of which participates in recombination in vivo, for induction of meiotic recombination in plants. The analysis was made of recA gene DNA sequence in silico, that was cloned and the chimeric genes were obtained in which the nucleotide sequences of recA reporter gene and sign of nuclear localization are fused in the frame. The complex molecular-biological analysis of expression of cloned genes in bacteria and functional analysis of properties of chimeric protein products were carry out. On the basis of obtained data the authors construct the native and chimeric genes under control of 35S CaMV promoter and agrobacterial strains for the plant transformation.