Development and study of recombinant inbred lines of sunflower with different content of oleic acid in seed oil

The creation and study of recombinant inbred lines is an effective method in breeding and genetic work with polygenic traits. In our study, a similar approach was used to indicate the content of oleic acid in sunflower seed oil. The experiments were carried out in the field and laboratory conditions in VNIIMK, Krasnodar, in the period 2012-2020. To obtain recombinant inbred lines with different contents of oleic acid in seed oil, hybridization, self-pollination and disruptive selection were used for three reciprocal combinations LG27 × LG28, LG27 × VK678 and LG27 × LG26. The LG26 line has a high oleic phenotype, LG27 has a mid-oleic, VK678 has an increased oleic and LH28 has a low oleic. The analysis of the fatty acid composition of sunflower seed oil was carried out using the method of gas-liquid chromatography of methyl esters on Chrom-5 and Chromatek-Crystal 5000 instruments. Molecular genetic marking of high oleic sunflower samples was performed using two pairs of primers: F13/R5 and F4/R1...