Creation of a symbiotic starter culture and study of its biochemical properties

In order to expand the range of functional fermented dairy bioproducts, various microbial monocultures and their symbiotic combinations are used. The aim of the work is to create a production symbiotic starter culture based on propionic acid bacteria of the Propionibacterium freudenreichii Sh-85 strain and kefir fungal starter culture. The symbiotic coexistence of the microflora of kefir fungal starter culture and propionic acid bacteria has been proven. A hypothetical scheme of interaction of microorganisms in a symbiotic starter culture has been developed. Lactate formed during lactic acid fermentation is used by propionic acid bacteria as a food source. Propionic bacteria enhance the usefulness of the medium by enriching it with metabolic products, in particular, B vitamins, and the microflora of kefir fungal starter culture uses these growth factors for its development. The dose of the applied laboratory starter culture for the production of a symbiotic starter culture was determined to be 5 %, the number of viable cells of propionic acid bacteria at the same time is 109 к.о.е./cm3. The description of the scheme of preparation of a symbiotic industrial starter culture is given. The biochemical properties of laboratory and industrial symbiotic starter cultures have been studied. The level of biochemical synthesis of vitamin B12 in symbiotic starter cultures has been established. Studies have been conducted confirming the long shelf life of the symbiotic production starter culture. Thus, a production symbiotic starter culture based on kefir fungal starter culture and propionic acid bacteria with high biochemical activity has been developed, which can be used for the production of a new fermented dairy bioproduct.