The method of controlling the extensiveness and quality of hydrolysis of maral raw materials by addition of organic solvents

The assessment of the influence of organic solvents on the extensiveness and quality of fermentative hydrolysis of raw materials of marals was carried out. As a model substratum the sinews of the maral previously crushed by means of industrial grinder of MIM-300 were used. The hydrolysis was carried out with addition of the complex of enzymes Protozyme B, Protozyme C and Protozyme LP ezymes at the temperature of 50°C in Elmasonik ultrasonic unit with water module of 1:5 for 4, 6 and 8 hours and with the addition of ethyl alcohol at the concentrations of 1, 3 and 6 % and glycerol at concentrations of 0.5, 1 and 3 %. In each test biochemical structure, the amount of aminous nitrogen and biological activity by the standard techniques were determined. It was shown that fermentative hydrolysis of raw materials of marals in the presence of organic solvents influenced the quality indicators of ready hydrolysates. Addition of ethanol in the concentration of 1 % when carrying out fermentation promoted the increase in the mass fraction of proteinaceous component to 91.4 %. Carrying out hydrolysis in the presence of ethyl alcohol in the concentration of 1 % promoted the increase of biological activity of hydrolysates which resulted in the increase in the duration of swimming of mice with freight at 93.3 % in comparison with control. The hydrolyzates from raw materials of marals received in the presence of glycerin as organic solvent were characterized by the decrease in the mass fraction of protein by 4.9-35.4 % in the presence of extractive substances without nitrogen in comparison with the hydrolyzates received in ethanol presence. It was established that the concentration and character of organic solvent had had the impact on the depth of fermentative hydrolysis. The maximum indicator of aminous nitrogen of 205 mg% was observed at fermentation in the presence of onepercentage ethanol.