Comparative evaluation of the level of nerve growth factor in experimental models of interstitial cystitis / bladder pain syndrome

Aim - to evaluate levels of nerve growth factor (NGF) in blood and urine in different experimental models of interstitial cystitis / bladder pain syndrome (IC / BPS). Material and methods. IC / BPS was modeled on white New Zealand female rabbits with 1500-2000 g body mass using different techniques: group I (n=8) - injection of 70% alcohol solution into bladder cavity; group II (n=7) - injection of protamine sulfate into bladder cavity; group III (n=8) - injection of 0,5% solution of hydrochloric acid (HCl) into urinary bladder; group IV (n=15) - injection of urine taken from an animal's bladder into bladder wall; group V (n=7) - injection of 0,9% sodium chloride (NaCl) solution into bladder wall; group VI (n=8) - intact rabbits (control group). Nerve growth factor was measured in blood and urine of animals using enzyme immunoassay (ELISA) 1 day and 14 days after the beginning of experiment. Results. At the 1st day of experiment blood level of NGF in all groups was statistically significantly higher than in control group, while only in group IV its level was elevated in urine (by 68,2%, p