Comparative assessment of the possibility of cellular material collection to matrixes obtained by methods of electrospinning and aerodynamic formation in a turbulent gas flow
Автор: Afanasiev S.A., Muslimova E.F., Nashchekina Yu. A., Nikonov P.O., Rogovskaya Yu. V., Bolbasov E.N., Tverdokhlebov S.I.
Журнал: Сибирский журнал клинической и экспериментальной медицины @cardiotomsk
Рубрика: В помощь практическому врачу
Статья в выпуске: 3 т.32, 2017 года.
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Introduction. Methods of electrospinning and aerodynamic formation in the gas stream allow the fabrication of synthetic structures similar to intercellular matrices. But it is important to assess the suitability of such structures for colonization by cellular material. Materials and Methods. Porous matrices of polylactic acid are used. Matrix No. 1 was obtained by the method of electrospinning, matrix No. 2 - by the method of aerodynamic formation. The structure of the matrices was examined on an electron microscope. The matrices were dynamically populated with stromal cells from the rabbit’s bone marrow, then matrices were cultured for 3 days in a CO2 incubator at 37 °C. Cells were detected by fluorescence microscopy. Data were presented as mean ± standard deviation. Results. The matrices differed in structure. Matrix No. 1 was formed by fibers of regular cylindrical shape (diameter 1.5±0.7 μm) without its own relief. The average porosity was 67±3%. In the structure of matrix No.2 it was possible to single out a macrolevel represented by wisps (diameter 27.5±17.4 μm) formed by directed fibers (diameter 0.44±0.14 μm) with developed surface relief. The average porosity was 55±3% (p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }
Electrospinning, aerodynamic formation, cell culture, nonwoven materials
Короткий адрес: https://sciup.org/149125174
IDR: 149125174 | DOI: 10.29001/2073-8552-2017-32-3-71-76