Structure of oil flax cultivar Y 117 based on the polymorphic microsatellite loci

Oil flax is a high-demand highly profitable crop. The modern standards of breeding and seed growing expect to the genetic passportization of the breeding achievement. Microsatellite sequences of DNA are an effective instrument to conduct the passportization of the genetic material. Oil flax cultivars-self-pollinators are known to be able to possess the intravarietal heterogeneity. That’s why using a system of microsatellite DNA-markers, we estimated the genetic structure of the cultivar Y 117 of oil flax bred at the V.S. Pustovoit All-Russian Research Institute of Oil Crops. The estimation of the genetic structure was conducted in a series of 40 plants. We used 16 microsatellite markers in our work. The analysis of PCR products showed the series of plants is homogenous by 13 loci. Loci Lu3, Lu21, and Lu27b had two alleles. The analysis by three polymorphic loci of allele state of the separate plants of the cultivar reviled four groups of plants having a different type of allele combination (biotypes): A (Lu3175Lu21´109 Lu27b135), B (Lu3166Lu21´117Lu27b142), C (Lu3166Lu21´109Lu27b135), and D (Lu3175,166Lu21´109 Lu27b135) with a frequency of 57.5%, 37.5, 2.5, and 2.5%, respectively. Our results certifies that the cultivar Y 117 can be considered as a complex of biotypes. The main biotypes A and B totally reach 95% of the series. The biotypes D and C (totally 5% of the series) possibly are a result of pollination between plants of biotypes A and B. In a view of origin of this cultivar from some unique by the resistance to flax sickness plants, the amount of reviled biotypes is little. However taking in to attention an admissible level of allogamy within a cultivar, with time a shift in a combination of biotypes and some increase in their amount is possible. The combination of biotypes can be used as a parameter to control the stability of a cultivar, which is one of the definitive criteria of a cultivar according to the requirements of the International Union for the Protection of New Varieties of Plants (UPOV.