Structural and functional analysis of manganese- and zinc-dependent proteins in plants growing in the south of Russia

Modern bioinformatic approaches allow structural and functional analysis of proteins of various organisms for the presence of homologous and non-homologous sites. This study is aimed at identifying structural and functional differences of manganese- and zinc-dependent proteins in common plant species growing on the territory of arid biocenoses of Southern Russia. The main purpose of the work was to study the presence of manganese- and zinc-dependent proteins included in the proteome of common plant species typical of arid territories of Southern Russia. As a result of the analysis of the plant proteome for the presence of manganese- and zinc-dependent proteins, it was found that the degree of study was very low, the number of annotated proteins was 5 pieces only for Artemisia annua and Artemisia lercheana , while there was no protein annotation at all for other species. Later, using a bioinformatic search, the presence of a manganese-permeable Photosystem II protein D1 protein and a zinc-dependent Acetyl-CoA carboxylase carboxyl transferase subunit beta was found in all plants. Structural analysis of sequences using multiple alignment showed that there are strong structural differences in the manganese-dependent Photosystem II protein D1 protein between Artemisia lercheana, Artemisia annua, Cichorium intybus, Descurainia Sophia, Tripleurospermum inodorum and Xanthium albinum , and for the zinc-dependent protein Acetyl-CoA carboxylase carboxyl transferase subunit beta in Artemisia lercheana, Artemisia annua, Cichorium intybus and Descurainia sophia , which is associated with a variety of amino acid substitutions, including conservative and weakly conservative sites. The obtained results of the study showed a low degree of knowledge and description of the structural and functional properties of metal-dependen.