Technology of obtaining of enzymic forage additives based on vegetable raw materials

An important scientific and practical task of biotechnology is the development of complex feed additives with enzymatic properties that can improve the digestibility of hard-to-hydrolyze feed components. A promising direction is the production of feed additives based on vegetable raw materials. The aim of the research was to develop an enzyme feed additive with cellulolytic properties based on plant raw materials using an enzyme complex of a fungus of the genus Trichoderma , to determine its toxicity on protozoa and warm-blooded animals. To obtain an enzyme feed additive from cellulose-containing waste, we used a 72-hour liquid mother culture of the microscopic fungus Trichoderma atrobrunneum VKPM F-1434 from the educational collection of the Department of Biotechnology of the Orel State Agrarian University, which was grown on a sucrose-yeast medium (SD medium) enriched with sprouted crushed and dried grain with the addition of glycine. Cellulose-containing agricultural waste was used as the basis of the feed additive: a mixture of coarse wheat bran and wheat straw (in a ratio of 5:2). The resulting biologically active enzyme feed additive is characterized by a reduced content of" raw "fiber (18%), an increased content of" raw " protein (34%). The cellulolytic activity of the feed additive is 72.4 u/ml. An enzyme feed additive based on plant raw materials is an absolutely non-toxic product, in this regard, it can be recommended for use in industrial animal husbandry in order to increase the productivity of farm animals.