The effect of some different blood anticoagulants on rabbits plasma biochemical parameters under different temperatures

The aim of present study was to compare the effect of Ca chelat-ing anticoagulants (EDTA, citrate and oxalate) and antithrombin anticoagulant (heparin) on some rabbits plasma biochemical pa-rameters at room temperature 25 °С and extreme temperatures -18 °С and 50 °С for 1hr. Five male rabbits weighting between 800-1000gm, kept in the animal house /College of Veterinary Medicine/University of Diyala, under 12:12 dark light cycle with ad libtum food and water in individual cages (60cm,40cm) during November and December /2022. Four ml of blood samples were collected by cardiac puncture (23 G needle) into four tubes con-tain anticoagulants: (EDTA, citrate, oxalate and heparin)one ml of blood for each tube; then the blood samples were kept at :room temperature 25 °С, waterbath 50 °С and refrigerator -18 °С, for 1hr then put in centrifuge at 3000 rpm for 15 min. From total ex-amined blood samples =24 for each Ca chelating anticoagulants (EDTA, citrate and oxalate) and antithrombin anticoagulant (hep-arin) after 1hr under in 25 °С, -18 °С and 50 °С. The both groups of anticoagulants (Ca chelating and antithrombin) showed no significant differences P>0.05 between them in level of biochem-ical parameters (glucose, total protein, cholesterol and urea) after 1 hr at 25 °С; on the other hand significant increase in plasma glucose, total protein and cholesterol beside significant decrease in plasma urea of Ca chelating anticoagulants after 1hr at -18 °С and 50 °С as compare with antithrombin anticoagulant. In con-clusion; heparin was more effective than Ca chelating anticoag-ulant in preserve plasma biochemical parameters within normal values under extreme cool and hot condition