Glutathione levels in erythrocytes and blood plasma of cows patients with endometritis

In this paper we studied the blood of cows 2-3 years old black-and-white breed. For further biochemical studies using plasma and hemolysate washed erythrocytes. The content of reduced glutathione (GSH) was determined by forming a complex with 5,5-dithio-bis-(2-nitrobenzoic) acid. All cows were divided into 4 groups: group 1 (control) -klinicheski healthy animals, the first half of pregnancy; group 2 - cows diagnosed with acute postpartum endometritis, group 3 - purulent-catarrhal endometritis, group 4 - animals without clinical signs of endometritis. Vyyavleno significant increase in glutathione content of erythrocytes and plasma of cows diagnosed with acute postpartum endometritis, and in patients with more severe animal form of the disease - purulent-catarrhal endometritis-naprotiv, glutathione levels in erythrocytes decreased in comparison with the control group. The content of reduced glutathione is retained in blood plasma increased in patients post-partum animals of this group. In treated animals without clinical signs of endometritis 25-30 days after calving revealed no significant changes in the content of glutathione in erythrocytes and plasma in comparison with the control group of animals. Changes in the content of glutathione as an essential component of the antioxidant defense is probably due to the development of oxidative stress and the intensification of free radical processes. Indicators of the level of glutathione in erythrocytes and blood plasma may be an additional criterion for the effectiveness of treatment of postpartum endometritis in cattle.