Conditions and periods of storage of the different preparation forms of microbiopreparations based on perspective strain-antagonists of a white rot pathogen

Xk-1-4 Chaetomiumolivaceum and B-12 Bacillus licheniformis - the strains actively decomposing sclerotia of a white rot on rapeseed and possessing a growth stimulating activity to a rapeseeds - were selected by graded screening from a collection of the laboratory of biological methods of VNIIMK. Conditions and periods of storage of preparation forms liquid culture and powder in two modifications were determined to develop elements of the technological regulations for production of microbiopreparations on a base of these strains. A liquid culture based on a strain of a fungus Xk-1-4 Chaetomiumolivaceum can be kept with a titer on a level 1.0-9.0 x 10 6 KFU/ml within two months both in fridge and at variable temperature adding 0.1% ascorbic acid, 5.0% PVP and 10.0% sodium humate. The best preparation form for a microbiopreparation based on a strain of a fungus Xk-1-4 Chaetomiumolivaceum is a powder on perlite, developed by spore washout from a hard nutrient medium, further mixed withfiller, keeping a titer at variabletemperature on a level 10 7 KFU/ml within nine months. Compared to a fungus preparation, a liquid culture of microbiopreparation based on B-12 Bacilluslicheniformiscan be kept at variable temperature during six months with a titer holding the level of 10 10 KFU/ml adding 1.0% PVP or 10.0% sodium humate or 5.0 % glycerin. When using preparation from powder on perlitetiter of the preparation have been kept on the level 10 10 KFU/gat variable temperature within nine months.