Improving the technology of hydrolysis of marals’ raw materials

The effect of various concentrations of ethyl alcohol, glycerol and hypochlorite on the process of enzymatic hydrolysis of maral raw materials was studied. By-products of velvet antler deer farming (tendons, reproductive organs of stags, uteri with embryos and amniotic fluid) were used as the material. Hydrolysis was carried out in ultrasound installation at hydromodule 1: 5, 1: 10 at the temperature of 50 °C during 4, 6, 10 hours. The fermentation was carried out with using enzymes of microbic origin Protozyme B, Protozyme C and Protozyme LP, brought in solution in the number of 0.5 % of raw materials volume. It was found that the addition of ethanol in the amount of 1 % of water volume during the hydrolysis of maral raw materials led to the increase in the yield of dry substances compared to the control samples; specifically, the yield of dry substances increased by 2.1 times for the processing of tendons, by 23.8 % for the hydrolysis of uteri, and by 12.9 % for the fermentation of reproductive organs of stags. Using glycerin for the hydrolysis of tendons allowed obtaining the increase in the yield of dry substances by 2.0 times compared to the control samples, when the amount of glycerol was 3 %. According to the assessment of organoleptic properties of the concentrates, using ethanol at the concentration of 1 % was found to be optimal. It was shown that using low concentration of organic solvents at proteolysis of raw materials of marals allowed reducing extraction time by 1.3-1.6 times compared to the fermentation technology without ethyl alcohol. It was established that at raw materials fermentation in the presence of organic solvents the increase in the hydromodule led to the decrease in the exit of the concentrate, it was shown that hydromodule 1 had been optimum: 5.