Beta-lactam antibiotics effect on mitosis pathology at Allium test

The beta-lactam antibiotics (cefotaxime and augmentin) effect on mitosis’s pathology in the Allium test in the first metaphase after processing and after finishing the recovery period is investigated. Research methods: Allium test, cytogenetic analysis, statistical analysis. The experiment was conducted on a batch of the Stuttgarten variety which is genetically unstable as the result of micronucleus test. The results of aminoglycoside antibiotics on genotoxicity testing showed the lack of negative cefotaxime’s influence (in the concentration of 500.0 mg/l) on the mitosis’s pathology value in comparison with the control, regardless of action time/ valid time (during the 1 or 2-3 cell cycles). The increase of pathological processes was observed when testing augmentin (in the concentration of 800.0 mg/l). Under the influence of augmentin the percent of micronucleus cells increased from 0.3% (as a control/ in a control) to 2.3% (in the experiment). Also, in comparison with the control, the value of mitosis’s pathology increased from 6.1% (as a control/ in a control) to 10.1-16.0% (in the experiment). There is a significant increase of cells with bridge / cells bridge and mitosis. C-mitosis was observed in recovery period variants after applying augmentin (in the concentration of 800.0 mg/l, on exposure of 48 hours) and after cefotaxime (in the concentration of 500.0 mg/l, on exposure of 48 hours), in the first case as a typical mitosis in 20% of the cells with PM (pathology mitosis), in the second case - chromosome discharge in the metaphase and the anaphase of the 30% cells with PM. When testing augmentin in the first metaphase of the cell period, chromatic agglutination (12%), chiasma and chromatid bridges (35%) were observed. It is indicated that augmentin (in the concentration of 800.0 mg/l, on exposure of 48 hours) shows genotoxicity in comparison with the control and it’s able to change the rate of entry of onion cells into mitosis and the normal course of division processes. The recovery period for this antibiotic does not reduce the number of cells with mitosis pathology, but it changes their composition and spectrum.