Influence of Carbon Source on the Accumulation of Biomass by Cellulolytic Microorganisms as Producers of Feed Additive

The article presents the results of a study on the selection of components and optimization of the composition of nutrient media for the cultivation of cellulolytic microorganisms. The efficiency of microbial biomass accumulation in 19 strains has been evaluated using spectrophotometry in three variants of media differing in their carbon source. It has been found that the medium with a recalcitrant carbon source provides slow but stable growth, whereas the medium with an easily accessible carbon source induces rapid but unstable growth followed by metabolic exhaustion. The most effective medium has proved to be the combined one, which ensures both high initial growth rate due to readily available lactose and longterm stability due to poorly hydrolyzable carboxymethyl cellulose (CMC), making it optimal for the cultivation of cellulolytic microorganisms.