Effect of ram mesenchymal stem cell (MSC) lysate on sperm quality indicators

The aim of the study was to investigate the effect of lysate (L) of mesenchymal stem cells (MSC) from adipose tissue (AT) and bone marrow (BM) of rams on the qualitative parameters of spermatozoa during three-hour incubation. Three groups of samples were formed with 10 samples in each: control (sperm 7 • 1010 cells/ml + PBS buffer 100 μl, pH - 7.4), the first experimental (sperm 7 • 1010 cells/ml + lysate (L) of MSCs BM 2 • 108 cells/ml, pH - 7.2), the second experimental (sperm 7 • 1010 cells/ml + lysate (L) of MSCs AT 2 • 108 cells/ml, pH - 7.2). In order to establish the degree of sperm survival in media containing mesenchymal stem cells and to determine their effect on sperm quality, a four-stage sperm quality assessment was performed: 0; 1; 2 and 3 h after sperm incubation with mesenchymal stem cell lysate at 37 °C. Values were compared at each incubation stage (0; 1; 2 and 3 h) within each group. Differences were considered reliable at p ≤ 0.01 and p ≤ 0.05. Incubation of ram sperm in media containing lysate-mesenchymal stem cells from adipose tissue and bone marrow for 3 hours at a temperature of 37 °C contributes to reliable preservation of sperm viability with the following morphofunctional characteristics: progressiveness of movement - (43.10 ± 6.86) % (p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }