Effect of presowing seed treatment with microbial preparations on seed germination and accumulation of photosynthetic pigments in Trifolium pannonicum leaves

The influence of pre-sowing seed treatment with one-, two- and three-component microbial preparations containing Rhizobium trifolii, Fischerella muscicola, Trichoderma sp., on the field germination of seeds, the accumulation of photosynthetic pigments in leaves and the yield of Pannonian clover (Trifolium pannonicum Jacq.) of the first year of vegetation was studied. The greatest stimulating effect on seed germination was exerted by microbial suspensions containing Rh. trifolia. When seeds are treated with microbial suspensions containing Rh. trifolii (Rh. trifolii, Rh. trifolii + Trichoderma sp., Rh. trifolii + F. muscicola + Trichoderma sp.), a more powerful photosynthetic apparatus with a high content of chlorophylls and carotenoids was formed. Seed inoculation with one- and two-component biological products containing Trichoderma sp. caused a decrease in the level of chlorophyll in clover leaves. Microbial suspensions containing F. muscicola (F. muscicola and Rh. trifolii + F. muscicola) had no effect on the pool of green pigments in clover leaves. A strong positive correlation (r = 0.72) between the accumulation of biomass by clover plants and the content of chlorophylls in leaves was revealed. The three-component biopreparation containing Rh. trifolii + F. muscicola + Trichoderma sp. Microbial suspensions of Rh. trifolia, Rh. trifolii + Trichoderma sp., Rh. trifolii + F. muscicola + Trichoderma sp. showed their effectiveness on Pannonian clover plants and can be used for the production of biological products that increase the productivity of agrocenoses.